| Instructors | Brian Bowen, Ruth Gates, Steve Karl, Teresa Lewis, Mike Rappe, Rob Toonen | ||||
| Workshop will start each morning at 9 a.m. unless otherwise scheduled in advance. | |||||
| Monday | 14 | Morning | Lecture | Welcome and Introduction of Instructors Student PowerPoint presentations Introduction to Hawaiian Corals/Kaneohe Bay Introduction to Lab and Lab Safety |
All Cox? Lewis |
| Afternoon | Snorkel | Collection of samples, set up for DNA extractions | Toonen | ||
| Tuesday | 15 | Morning | Lecture/Lab | Nucleic acids, complete DNA extraction | Toonen |
| Afternoon | Lecture | Electrophoresis I | Rappe | ||
| Wednesday | 16 | Morning | Lecture | Population genetics overview and analysis | Bowen, Toonen |
| Afternoon | Lab/Lecture | PCR theory, PCR and sequencing | Toonen | ||
| Thursday | 17 | Morning | Lab | Electrophoresis II, PCR products and electro4 demos (PFGE, DGGE) |
Rappe |
| Afternoon | Demo | Sequencing (Sarah Daley) | |||
| Friday | 18 | All day | Lab/Lecture | RFLP/DGGE | Gates |
| Saturday | 19 | Morning | Lecture | Microsatellites | Toonen |
| Afternoon | Lab | Analysis of microsatellite data | Toonen | ||
| Sunday | 20 | Morning | Hanauma Bay trip | ||
| Afternoon | Snorkel | Collect K-Bay samples and freeze for extractions | |||
| Monday | 21 | Morning | Lecture | Bioinformatics | |
| Afternoon | Lab | Computer lab | |||
| Tuesday | 22 | Morning | Lecture | Functional genomics | Gates |
| Afternoon | Lab | RNA extraction, spec analysis, cDNA synthesis | Lewis | ||
| Wednesday | 23 | Morning | Lecture/Lab | QPCR | Lewis |
| Afternoon | Lab | Cellular disaggregation techniques | Lewis | ||
| Thursday | 24 | Morning | Lecture/Lab | Cellular Diagnostics, protein extraction and assays | Lewis |
| Afternoon | Lab | Protein gel electrophoresis and membrane transfer | Lewis | ||
| Friday | 25 | Morning | Lab | Immunoblot | Lewis |
| Afternoon | Lab | Lab clean up and exit survey | Lewis | ||
| Evening | Pau Hana Party | ||||
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