Faculty Projects and Publications

Peer-Reviewed Journal Articles

(2000-present)

Johnson, M.C., B. E. Simon, C.H. Kim, and J. C. Leong. 2000. Production of recombinant snakehead rhabdovirus (SHRV): the NV protein is not required for viral replication. J. Virology 74:2343-2350.

Kim, C. H., M. C. Johnson, J. D. Drennan, B. E. Simon, E. Thomann, and J. C. Leong.  2000.  DNA Vaccines encoding viral glycoproteins induce non-specific immunity and Mx protein synthesis in fish.  J. Virology 74: 7048-7054.

Chiou, P. P., C. H. Kim, P. Ormonde, and J. C. Leong. 2000.  The infectious hematopoietic necrosis virus matrix protein inhibits host-directed gene expression and induces morphological changes of apoptosis in cell cultures.  J. Virology 74:7619-7627.

Nygaard, R., S. Husgard, A-I. Sommer, J. C. Leong, and B. Robertsen.  2000.  Induction of Mx protein by interferon and double-stranded RNA in Salmonid cells.  Fish & Shellfish Immunology 10:435-450. 

Simon, B., J. Nomellini, P. Chiou, W. Bingle, J. Thornton, J. Smit, and J. Leong.  2001.  Recombinant vaccines against infectious hematopoietic necrosis virus:  production by the Caulobacter crescentus S-layer protein secretion system and evaluation in laboratory trials.  Dis. Aquat. Org. 44:17-27. 

T. L. Crippen, L. M. Bootland, J. C. Leong, M. S. Fitzpatrick, C. B. Schreck, and A. T. Vella.  Analysis of Salmonid Leukocytes Purified by Hypotonic Lysis of Erythrocytes.  2001. Journal of Aquatic Animal Health 13:234-245.

Alonso, M. and J. C. Leong.  2002.  Suppressive subtraction libraries to identify interferon-inducibe genes in fish.  Marine Biotechnology 4:  74-80.

Alonso, M., M. Johnson, B. Simon, and J. C. Leong. 2003.  A specific expression vector containing the interferon regulatory factor 1A (IRF1A) promoter for genetic immunization of fish.  Vaccine 21: 1591-1600..

Milston, R.H., A.T. Vella, T. L. Crippen, M. S. Fitzpatrick, J. C. Leong, and C. B. Schreck.  2003. In Vitro Detection of Functional Humoral Immunocompetence in Juvenile Chinook Salmon (Oncorhynchus tshawytscha) using Flow Cytometry.  Developmental and Comparative Immunology 15: 145-58.   

Milston, R. H., M. S. Fitzpatrick, A. Vella, D. Undersen, G. Feist, S. Clements, T. Crippen, J. Leong, and C. B. Schreck.  2003.  Short term exposure of Chinook salmon (Oncorhynchus tshawytscha) to o, p’ DDE or DMSO during early life history stages causes long-term humoral immunosuppression.  Environ. Health Perspectives 111, 1601-1608.

Leisy, D., T.D. Lewis, J. C. Leong, and G. Rohrmann.  2003.  Transduction of cultured fish cells with recombinant baculoviruses.  J. General Virology 84: 1173-1178.

M. Alonso, C. H. Kim, M. C. Johnson­, M. Pressley, and J.C. Leong.  2004.  The NV gene of snakehead rhabdovirus (SHRV) is not required for pathogenesis and a heterologous glycoprotein can be incorporated into the SHRV envelope. J. Virology 78: 5875-5882.

I. Misumi, A.T. Vella, J.C. Leong, T. Nakanishi, and C.B. Schreck.  2005.  p,p’-DDE depresses the immune competence of Chinook salmon (Oncorhynchus tshawytscha) leucocytes.  Fish and Shellfish Immunology 19:97-114.

Hollingsworth, L.L., R.A. Kinzie III, D. A. Krupp, T. D. Lewis, and J. C. Leong.  2005. Phototaxis of motile zooxanthellae to green light may facilitate symbiont capture by coral larvae. Coral Reefs 24(4):523.

Alonso, M., D.A. Stein, E. Thomann, H. M. Moulton, J. C. Leong, P. Iversen, and D. V. Mourich.  2005.  Inhibition of infectious haematopoietic necrosis virus in cell cultures with peptide conjugated morpholino oligomers.  J. Fish Diseases 28 (7):399-410. 

Hagedorn, M., V.L. carter, R.A. Steyn, D. Krupp, J. C. Leong, R.P. Lang, and T. R.l Tiersch.  2006.  Preliminary studies of sperm cryopreservation in the mushroom coral, Fungia scutaria.  Cryobiology 52(3):454-8.

Hagedorn, M., R. Pan, E. F. Cox, L. L. Hollingsworth, D. A. Krupp, T. D. Lewis, J. C. Leong, P. Mazur, W. Rall, D. MacFarlane, G. Fahy, and F. W. Kleinhaus.  2006.  Coral larvae conservation physiology and reproduction.  Cryobiology 52 (1):33-47. 

Robertsen, B., J. Zou, C. Secombes, J. Leong.  2006.  Molecular and expression analysis of an interferon-gamma inducible guanylate-binding protein from rainbow trout.  Developmental & Comparative Immunology 30(11): 1023-1033.

Misumi, I., T. Yada, J. C. Leong, and C. B. Schreck. (2009)  The effect of in vitro exposure to tributylin on the immune competence of Chinook salmon (Oncorhynchus tshawytscha) leukocytes.  Archives of Environmental Contamination and Toxicology 56 (2): 229-237.

Palumbi, S.R., J. D. Allen, M. W. Beck, D. G. Fautin, M.J. Fogarty, B. S. Halpern, L.S. Incze, J. Leong, E. Norse, J. J. Stachowicz, D. H. Wall, and P. A. Sandifer.  2009.  Managing for Ocean Biodiversity:  Creating a National Biodiversity Agenda to Sustain Marine Ecosystem Services.  Frontiers in Ecology and the Environment 7(4):204-211..

Van Oppen, Madeleine JH, Jo-Ann Leong, Ruth D. Gates.  2009. Coral-virus interactions: a double-edged sword? Symbiosis 47 (1):1-8. .

Leong J C. 2009. Fish Viruses. Encyclopedia of Virology, 5 vols. (B.W.J. Mahy and M.H.V. Van Regenmortel, Editors), pp. 227-234 Oxford: Elsevier.

Leong, J. C., and G. Kurath.  2009.  Novirhabdoviruses.  The Springer Index of Viruses. (C.A. Tidona and G. Darai, Editors), online.  Springer-Verlag.

Work, T.M., J. Dagenais, G.H. Balazs, J. Schumacher, T. d. Lewis, J. C. Leong, R. N. Casey, and J. W. Casey.  2009.  In vitro biology of fibropapilloma-associated turtle herpesvirus and host cells in Hawaiian green turtles (Chelonia mydas).  Journal of General Virology 90:1943-1950.

Ackermann, M., M. Koriabine, P. J. De Jong, T. D. Lewis, U. Buchler,  T. M. Work, J. Degenais, G. H. Balazs, and J. C. Leong.  2008.  Detection of a gene encoding sialylytransferase in a BAC comprising the genome of the fibropapilloma-associate turtle herpesvirus.  Virology, in review.

M. Alonso, E. Thomann, I. Misumi, T. Vella, C. Schreck, and J. C. Leong. 2008.  Fish Mx protein:  a potential environmental biomarker.  Fish and Shellfish Immunology, in preparation.

Lewis, T.D., E. F. Cox, and J. C. Leong.  2007.  A method for cellular disaggregation and cell-type isolation in scleractinian corals.  Marine Biotechnology, in preparation.

Reports, Theses, Proceedings

(2000-present)

Leong, J. C., T. Crippen, J. Drennan, M. Johnson, D. Jordan, C. Kim, B. Simon, and E. Thomanne.  2000.  Development of DNA Vaccines for Fish.  Suisanzoshoku 48(2):285-290, 2000.  A New Paradigm for Aquaculture 3.  Proceedings of the International Symposium on the New Paradigm for Aquaculture.  Feb. 8-10, 2000.  Nippon Convention Center, Chiba, Japan.  Edited by H. Sugita, R. Ueda, K. Yoshihara, and N. Taniguchi.  Japan Aquaculture Society.

Hollingsworth, L. L., T. D. Lewis, and J. C. Leong.  2005.  Early onset and expression of fluorescent proteins in the larvae of the mushroom coral, Fungia scutaria Lamarck 1801.  Proceedings of the 10th International Coral Reef Symposium, Okinawa, Japan, June 28-July 1, 2004, published 2006; pp. 99-105.

Leong, J.C., and M.A. Rivera.  2006.  Quarterly Progress Report III.  January-March 2006.  Hawaii Institute of Marine Biology – Northwestern Hawaii Islands Coral Reef Research Partnership.  44 pp.

Leong, J. C., M.A. Rivera, and J. Barrett.  2006. Quarterly Progress Report IV.  April-June 2006.  Hawaii Institute of Marine Biology – Northwestern Hawaiian Islands Coral Reef Research Partnership.  34 pp.

Leong, J.C., and C. Wiener.  2007.  Semi-annual progress report VII.  November 2007.  Hawai‘i Institute of Marine biology –Northwestern Hawaiian Islands Coral Reef Research Partnership,

Books and Chapters

(2000-present)

E. D. Anderson and J. C. Leong.  2000.  Development of DNA Vaccines for Salmonid Fish.  In DNA Vaccines:  Methods and Protocols.  ed. D. Lowrie and R. Whalen.  Humana Press. London, U.K., pp.105-121.

J.C. Leong, D. Brown, P. Dobos, F.S.B. Kibenge, J. E. Ludert, H. Muller, E. Mundt, and B. Nichsolson.  Birnaviridae. 2000.  In the 7th Report of the International Committee on the Taxonomy of Viruses.

A.  Benmansour, C.H. Calisher, D. Dietzgen, R.-X. Fang, A.O. Jackson, G. Kurath, J. C. Leong, S. Nadin-Davies, R.B. Tesh, N. Tordo, and P. J. Walker. 2000. Rhabdoviridae.  In the 7th Report of the International Committee on the Taxonomy of Viruses.

C. Kim and J. C. Leong.  Fish Viruses. 1999  In Encyclopedia of Virology, Second Edition.  ed.  R. G. Webster and A. Granoff.  Academic Press, London, U.K, pp. 558-568.

B.E. Simon and J. C. Leong. 2003.  A Review of Recombinant Vaccines for Aquaculture:  Bacterial Systems for Vaccine Production and Delivery.  Chapter 16 in Agrobiotechnology and Plant Tissue Culture.  Ed. Sant S. Bhojwani and Woong-Young Soh, Oxford & IBH Publishing Co., New Delhi, India.

Leong, J.C., and G. Kurath.  2009.  Infectious Hematopoietic Necrosis Virus.  In J. C. Leong.  2003.  DNA Vaccination of fish:  transient expression and protection against pathogens.  Aquaculture:  Retrsopective and Outlook.  Proceedings of the Meeting, AIP Aquaculture Summit. November 4-7, 2002.  Honolulu, Hawaii

T. D. Lewis and J-A. C. Leong.  2004.  Viruses of Fish.  Chapter 2 in Current Trends in the Study of Bacterial and Viral Fish and Shrimp Diseases.  Molecular Aspects of Fish & Marine Biology, Vol. 3, pp. 39-81. ed. K. Y Leung.  World Scientific Publishing Co., Singapore.

J. C. Leong.  2007.  Fish Viruses.  In Encylopedia of Virology.  Ed.  Brian J. Mahy and Marc HV von Regenmortel.  Published by Elsevier online at http:// ScienceDirect.com.

Bootland, L.M. and J. C Leong.  2009.  Infectious Hematopoietic Necrosis Virus (IHNV).  In:  P. Woo, ed., Viral, Bacterial and Fungal Infections, Vol. II.  Ed. P.T. K. Woo and D. W. Bruno. CABI International Publishing, in press.

Reviews and Abstracts

(2000-present)

M. Yoshimizu, Y. Hori, T. Yoshinaka, T. Kimura, and J. Leong. 2000.  Evaluation of methods used for the surveillance and monitoring of fish health for risk assessment.  Abstract.  Proceedings of the meeting, “International Conference on Risk Analysis in Aquatic Animal Health” sponsored by OIE.  Feb. 2000, Paris.

J. Leong et al., 2000.  Development of DNA Vaccines for Fish.  Third International Symposium of the Japan Aquaculture Society.  The New Paradigm for Aquaculture.  Feb. 8-11, 2000 Mihama-ku, Chiba City. 

B. Simon and J. Leong.  2000.  Attenuated Bacterial Delivery system for DNA Vaccines.  8th International Symposium on Developmental and Comparative Immunology.  Cairns, Australia.  July 2-7, 2000.

M. Alonso and J. Leong.  2000.  Development of fish promoters for DNA vaccination.  8th International Symposium on Developmental and Comparative Immunology.  Cairns, Australia.  July 2-7, 2000.

E. Thomann and J. Leong. 2000.  Mx expression as a biomarker for immune competence in fish.  8th International Symposium on Developmental and Comparative Immunology.  Cairns, Australia.  July 2-7, 2000.

W. M. Contreras-Sanchez, M. S. Fitzpatrick, M. Alonso, C.B. Schreck, and J. C. Leong. 2000.  Identification of unique genes expressed during sex inversion of Nile tilapia (Oreochromis niloticus) induced by short immersions in the synthetic steroid Trenbolone Acetate.  International Symposium on Fish Endocrinology.  Sept. 2000 Aberdeen, Scotland.

R. M. Milston, A. T. Vella, T. Crippen, C. B. Schreck,, J. C. Leong, and M. S. Fitzpatrick.  2000.  In vitro detection of humoral immunocompetence of juvenile salmon by flow cytometry.  International Symposium on Fish Endocrinology.  Sept. 2000, Aberdeen, Scotland.

J. C. Leong, J. Drennan, M. Johnson, D. Jordan, C. Kim, B. Simon, and E. Thomann.  2000.  DNA Vaccination and Disease Control in Aquaculture.  International Marine Biotechnology Conference.  Townsville, Australia.  Sept. 29-Oct. 4, 2000.

J. C. Leong, M. Alonso, L. Bootland, T. Crippen, M. Fitzpatrick, D. Jordan, D. Leisy, R. Milston, Carl Schreck, B. Simon, E. Thomann, and Anthony Vella.  2000.  Biomarkers of Immune Induction in Fish.  International Congress of Veterinary Medicine.  October 27-29,2000.  Puerto Varas, Chile.

J. C. Leong, M. Alonso, L. Bootland, D. Jordan, D. Leisy, B. Simon, and E. Thomann.  2000.  DNA vaccines for IHNV and IPNV.  International Congress of Veterinary Medicine.  October 27-29, 2000.  Puerto Varas, Chile.

J. C. Leong, M. Alonso, D. Leisy, B. Simon, and E. Thomann.  Viral Diseases in Aquaculture:  Impact and Control Measures.  The Asia Pacific Conference on Plant Tissue Culture & Agriciotechnology, November 19-23, 2000.  Singapore

Leong, J. C. et al., 2001.  Genetic Vaccines for Aquaculture.  Aquaculture Biotechnology:  Interface for Maximum Impact.  March 5-7, 2001.  National Conservation Training Center.  Shepherdstown, West Virginia.

Schwindt, A.R., Leong, J. C., and Schreck, C.B.  2002.  Stress induced differential gene expression in the brains of juvenile steelhead trout (Oncorhynchus mykiss).  American Fisheries Society, New Orleans, LA.

Schwindt, A.R., Leong, J. C., and Schreck, C.B.  2002. Stress induced differential gene expression in the brains of juvenile streelhead trout (Oncorhynchus mykiss).  International Congress on the Biology of fish.  July 21-22, 2002.  University of British Columbia, Vancouver, Canada.

Misumi, I., T. D. Lewis, J. C. Leong.  2006.  Immmune Response of Fish Against Cryptocaryon irritans and Identification of An Antigenic Parasite Protein.  Joint Seminar 2006.  National University Cooperation.  Tokyo University of Marine Science and Technology.  October 13, 2006.