BioTechniques Workshop 2000
College of Tropical Agriculture and Human Resources

The College of Tropical Agriculture and Human Resources (CTAHR), University of Hawaii presents this biotechnology workshop as part of the Millennium Workforce Development project. The workshop will provide scientists with knowledge and hands-on experience with contemporary instruments and techniques to assist the development of biotechnology industries in the State of Hawaii.

Location: The workshop, the housing and the meals are all at the University of Hawaii Manoa campus. The housing and meals are on your own cost. The workshop will provide coffee, soft drinks and snacks.

Workshop: Agricultural Science Building 318, 1955 East-West Road.

Housing: Lincoln Hall, 1821 East-West Road, Honolulu, HI 968848-1821. The cost is approximately $42 per night per person.

Meals (on your own): Paradise Palms cafeteria on campus.

Registration Fees: None.

Workshop Dates: November 27 – December 8, 2000

Workshop Format and schedule: This is a ten-day workshop with hands-on exercises! There will be two-hour daily lectures; six-hour (or more) laboratories per day; ten different experiments with one experiment of your choice; and one-day tour to visit representative research laboratories on the Manoa campus.

Two to three persons per group for maximum four groups.

Lecture 8-10 am every day; laboratory 10:30 am – 5:30 pm every day except Sunday

Social program
: First day: registration (4-6 pm), reception (6-8 pm)

Instructors: Drs. Qing X. Li, and Dulal Borthakur.


To develop the ability to use contemporary instrumentation and biotechniques in experiments representative of those conducted in analytical biochemistry, environmental science and technology, and molecular biology.

To transfer and adapt techniques and knowledge gained back to your home company.

To develop a strong relationship between the University of Hawaii and companies for future technology transfer and collaborations.

Approach: Chemicals, biochemicals, DNA and proteins will be used as model molecules in the experiments to practice the techniques and skills. The experimental materials will include plant tissues, animal tissues, marine organisms, bacteria, soil, and water. The techniques include:

Protein assay,
Enzyme assay,
Immunoaffinity chromatography,
DNA sequencing,
Western blot,
Gel electrophoresis,
Gas chromatography (GC),
GC – mass spectrometry (GC-MS),
High performance liquid chromatography (HPLC),
Capillary electrophoresis (CE),
Supercritical carbon dioxide fluid extraction (SFE),
Pressurized fluid extraction (PFE), and
Use of (but not limited to) ultra-centrifuges, pH meters, various balances, autoclaves, spectrophotometers, lyophilizers, graduated cylinders and pipets.

Further Information:

Qing X. Li, or Dulal Borthakur
Department of Molecular Biosciences and Biosystems Engineering
University of Hawaii
Agricultural Science Building 218
1955 East West Road
Honolulu, HI 96822
Telephone: (808) 956-8384
FAX: (808) 956-3542

| UHM | HNEI | CTAHR |BioSystems |Comments and Questions |