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PHS POLICY ON HUMANE CARE AND USE OF LABORATORY ANIMALS CLARIFICATION REGARDING USE OF CARBON DIOXIDE FOR EUTHANASIA OF SMALL LABORATORY ANIMALS
Release Date: 17 July 2002
The Office of Laboratory Animal Welfare (OLAW) has determined a need to issue Public Health Service Policy on Humane Care and Use of Laboratory Animals (PHS Policy) guidance to Assured institutions clarifying current requirements regarding the use of carbon dioxide (CO2) as a euthanasia agent for small laboratory animals.
The Animal Care unit of the Animal and Plant Health Inspection Service, U.S. Department of Agriculture, has reviewed and concurs with this guidance as it relates to USDA-covered species.
For more information and clarification concerning the use of carbon dioxide visit