Oceanography Seminar - Byron Pedler SherwoodSeptember 4, 3:00pm - 4:00pm
Mānoa Campus, Marine Science Building 100
"Physiological and ecological constraints on bacterial turnover of marine dissolved organic matter--insights from a model system"
The ocean contains one of the largest reservoirs of reduced carbon on Earth in the form of dissolved organic matter (DOM). The objective of this study was to investigate the physiological and ecological constraints on microbial mediated DOM turnover by focusing on how a model heterotrophic bacterial strain makes a living in the sea. It was found that a single strain, Alteromonas sp. AltSIO, has the capacity to consume an equivalent magnitude of DOC as diverse bacterial communities, suggesting that bacterial diversity may not be required for the complete removal of labile DOC in the surface mesotrophic ocean. In long-term microcosms, however, bacterial diversity was required for continued degradation of semi-labile DOC. To test the generality of this capacity among individual bacteria, a culture-based study was conducted where >100 phylogenetically diverse bacterial strains were isolated to screen for growth in unamended ambient DOM. No other bacterial strain tested exhibited the capacity to consume a measureable quantity of DOC when grown in isolation, suggesting that this phenomenon may not be common among readily culturable marine bacteria. Physiological assays and genomic analysis of Alteromonas AltSIO demonstrated a broad capacity for processing carbohydrates and an apparent preference for disaccharides. DOM characterization by ultrahigh resolution mass spectrometry revealed that both AltSIO grown in isolation and diverse seawater communities significantly altered the chemical composition of ambient DOM after 40 days of incubation. These results will be discussed in the context of an evolving understanding of the relationship between microbial community composition and the lability of marine DOC.
Oceanography, Mānoa Campus
Sharrese, (808) 956-7633, email@example.com, http://www.soest.hawaii.edu/oceanography/index.html, Byron Pedler Sherwood (PDF)
Thursday, September 4
Volunteer and Engagement FairMānoa Campus, Hawaii Hall Lawn
College of Social Sciences FairMānoa Campus, Campus Center Courtyard
Current Situation of School Education in JapanMānoa Campus, Wist Hall, College Collaboration Center (CCC)
American Studies Final OralMānoa Campus, Moore Hall 323
Oceanography Seminar - Byron Pedler SherwoodMānoa Campus, Marine Science Building 100
Office of Public Health Studies Open House & Undergraduate Program LaunchMānoa Campus, Biomed D207