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My
research currently focuses on cell biological questions, particularly
on motility and the cytoskeleton. I am working with Drosophila
melanogaster as a model system because of the available genetic
and molecular data and because the vinegar fly can be genetically
manipulated in the laboratory. Recently, my laboratory has molecularly
characterized a gene which, when mutagenized, causes either
flightlessness or early embryonic lethality. Sequence analysis
has revealed that the gene product shares significant homology
with a class of human cytoskeleton-associated factors. My interest
in evolution concerns genetic and molecular events which may
have a profound effect on the phenotype of an organisms and
therefore focuses on developmentally important genes.
Other
associated interested include the question of genetic control
of neuronal pathfinding and neuromuscular connectivity during
development.
Methods
employed in my laboratory include standard molecular biological
techniques, in situ hybridization, monoclonal antibodies, immunocytochemistry
and genetic methods such as complementation testing, translocation
and deficiency analysis.
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Selected
Publications
| Maleszka,
R., H.G. de Couet and G. L. G. Miklos. 1998. Data
transferability from model organisms to human beings:
insights from the functional genomics of the flightless
region of Drosophila. PROC. NATL. ACAD. SCI. USA.
95: 3731-3736 |
| Maleszka,
R., S. D. Hanes, R. L. Hackett, H.G. de Couet, and
G.L.G. Miklos. 1996. The Drosophila melanogaster DODO
gene, conserved in humans, is functionally interchangeable
with the ESS1 cell division gene of Saccharomyces
cerevisiae. PROC. NATL. ACAD. SCI. USA. 93:447-451 |
| de
Couet, H. G., K.F. Fong, A. G. Weeds, P. Mc Laughlin,
and G.L.G. Miklos. 1995. Molecular and mutational
analysis of a gelsolin-family member encoded by the
flightless-I gene of Drosophila melanogaster.GENETICS
141:1049-1059 |
| Millard,
D. and H.G. de Couet. 1995. Preparation of glassplates
for DNA sequencing with cerium oxide. BIOTECHNIQUES
19: 576 |
| Kimura,
C., G.A. Ahearn, L. Busquets-Turner, S.R. Haley, C.
Nagao, and H.G. de Couet. 1994. Immunolocalization
of an antigen associated with the invertebrate electrogenic
2/Na+/1H+ antiporter. J. Exp. Biol. 189: 85-104 |
| Campbell,
H.D., T. Schimansky, C. Claudianos, N. Ozsarac, A.
B. Kasprzak, J. N. Cotsell, I. G. Young, H.G. de Couet,
and G.L. G. Miklos. 1993. The Drosophila melanogaster
flightless-I gene involved in gastrulation and muscle
degeneration encodes gelsolin-like and leucine-rich
repeat domains, and is conserved in Caenorhabditis
and human. Proc. Natl. Acad. Sci. USA 90: 11386-11390 |
| de
Couet, H. G. , A. Gresham, E. Lemme, and G. A. Ahearn.
1993. Electrogenic 2/Na+/1H+ exchange in a crustacean
epithelium is inhibited by a brush border monoclonal
antibody. Am. J. Physiol. 264: 804-810 |
| Bastiani,
M. J. , de Couet, H.G., Quinn, Karlstrom, R. O., J.
M. A., Kotrla, K., Goodman, C. S., and Ball, E. E.
1992. Position-specific expression of the annulin
protein during grasshopper embryogenesis. Development.
154: 129-142 |
| Milkos,
G.L.G., and H.G. De Couet. 1990. The mutations, previously
designated as flightless-I3, flightless-02 and standby
are members of the W-2 lethal complementation group
at the base of X-chromosome of Drosophila melanogaster.
J. Neurogenet. in the press. |
| Sandeman,
D.S., R. Sandeman, and H.G. de Couet. 1990. Extraretinal
photoreceptor cells in the brain of the crayfish Cherax
destructor. J. Neurobiology, in the press. |
| de
Couet, H.G., and T. Tanimura. 1987. Monoclonal antibodies
provide evidence that for opsin in the outer rhabdomeres
of Drosophila melangogaster is not glycosylated. Eur.
J. Cell Biol. 44: 50-56. |
| de
Couet, H.G., J. Davies, V. Pirrotta, and G.L.G. Miklos.
1987. Genetic and molecular studies of a flightless
mutant of Drosophila melanogaster. J. Neurogen. 4:
132-133. |
| de
Couet, H.G., S. Stowe, and A.D. Blest. 1984. Membrane-associated
actin in the rhabdomeral microvilli of crayfish photoreceptors.
J. Cell Biol. 98:834-846. |
| Vandekerckhove,
J., H.G. De Couet, and K. Weber. 1983. Molecular evolution
of muscle-specific actins: a protein-chemical analysis.
In: Structure and Function of Actin in Muscle and
Non-Muscle Cells, dos Remedios, Ch., Barden, J., Eds.,
pp 241-248. Melbourne: Academic Press. |
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