Educational Programs at the Institute

Histotechniques and Histology of the Anthozoa

Introduction • Course Schedule • Draft Course Syllabus

Course Schedule

 

Monday Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday Wednesday Thursday
23 Aug 24 Aug 25 Aug 26 Aug 27 Aug 28 Aug 29 Aug 30 Aug 31 Aug 1 Sep 2 Sep
8:00 Welcome to HIMB - Leong  Introduction to the Course - Aeby, Price, Peters Qualitative and Quantitative Field Methods - Aeby Basic Anatomy and Histology of the Anthozoa - Peters Change decal First group  puts on tissue (8 - 1), then goes to microtomes. Third group embeds; microtomy practice or slide reading Microtomy practice or slide reading Staining - Price Boat trip to northern Kanehoe Bay for snorkeling Slide reading: course samples - Peters Students leave
8:30 Discuss processing (if not covered earlier);  microtomy.  
9:00 Microtomy lecture  
9:30    
10:00                    
10:30 Hawaiian Marine Habitats and Anthozoans - Aeby  Kanehoe Bay Story - Cox Wash Helly's fixed tissues Post fixation procedures: enrobing, decalcification, cutting, orientation, processing. Slide reading: comparative aspects - Peters Histopathology of Pacific Anthozoans - Work Introduction to Histopathology - Peters Staining - general and special - and coverslipping Staining - Price Slide reading: course samples - Peters  
11:00  
11:30 Aeby continued  
12:00                  
12:30                      
1:00 Fixation and Collection damage and orientation - Price Snorkel to collect qualitative and quantitative field data - Aeby, Peters (species IDs, transect comparisons) Comparative Histology of Anthozoans - Peters Check decal progress, change, trim, wash, store, etc. Second group puts tissue on (2-7) Microtomy practice or slide reading Free time  Staining - Price   Slide reading: course samples - Peters  
1:30    
2:00 Snorkel: qualitative obs.;  collect specimens for fixation  Slide reading: Pacific anthozoans - Work    
2:30      
3:00            
3:30 Lab work on enrobing, decal, cutting Fun snorkel for exercise? Microtomy practice or slide reading Fun snorkel for exercise? Staining - Price CATCH UP TIME Course Wrap-up - Aeby, Price, Peters  
4:00  
4:30  
5:00 Tissues put into fixatives    
5:30                 End of Course Potluck Dinner  
6:00                  
6:30                  
7:00 Fixation of Anthozoan Tissues - Price Guest speaker Jean Kenyon, Methods of Field Assessment Analysis of Field Data - Aeby (teams work on their data sets, discussion) Guest speaker Ginger Garrison, African Dust Hypothesis Second group embeds; then sections Diseases of Anthozoans - Peters Microtomy OR slide reading CATCH UP TIME Slide reading: course samples - Peters  
7:30    
8:00    
8:30    
9:00 Put tissues on to wash Third group puts tissue on  (overnight)    
  Change decal              

 

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