2008 Edwin W. Pauley Summer Program in Marine Biology

Hormones and the Environment

July - August, 2008

The 2008 Edwin W. Pauley Summer Program in Marine Biology, entitled “Hormones and Environment”, focused on developing a better understanding of the mechanisms by which marine vertebrates are able to adapt to changing environments and environmental conditions and developing techniques to measure these adaptations both qualitatively and quantitatively.  An important component for our consideration was how environmental salinities affect teleosts on the levels of the cell, organ, and whole organism.

Thirteen visiting researchers and 18 graduate, undergraduate students and post-doctoral fellows from Japan and the U.S. participated in the 24th Annual Edwin W. Pauley Foundation Summer Program in Marine Biology at Coconut Island.  Besides providing opportunities for students to conduct research in environmental physiology of marine animals, we had three major goals.  The first goal was to review current information and discuss future research directions. This goal was met through attendance of 20 high quality lectures covering cutting edge research on a variety of fields including islet cell transplant for diabetes research, large scale breeding of hybrid striped bass, passive tracking of hammerhead sharks, and cellular adaptation to changing salinities in several organs and organisms.  Our second goal was to provide students with the cognitive and technical skills that will enable them to thrive in their profession.  This goal was met by a variety of different workshops throughout the summer; these included establishing ovulation stage and manual stripping of female tilapia, developing a novel technique to cannulate the esophagus of tilapia, comparative anatomy of reef fishes, gen cloning techniques, and establishment of a reporter gene assay system.  Also, the graduate and undergraduate students participated in several on going research projects that were being conducted throughout the summer.  Our last goal was to advance the state of knowledge through the publication of results of student research in peer-reviewed scientific journals.  We have 3 original papers either in press or submitted and 3 (Lori feed, Jason, Kai) original papers in preparation as a result of the experiments conducted during the summer program.  Furthermore, we have 4 (Lori, Andre, Jason) follow-up experiments in progress.

We thank the Pauley Foundation for their generous and continuing support of this summer program.  The Pauley funds helped support the research as well as travel, housing costs and meal allowance for all visiting faculty and students.  Dr. Stephen Pauley covered the travel expenses for two students from Pomona College.  In addition, several participants were able to cover their own travel expenses.

E. Gordon Grau, Ph.D.
Director of Hawaii Sea Grant
Professor of Zoology



Dr. Lori Davis, Hawaii Institute of Marine Biology, University of Hawaii, Manoa, USA
Dr. E. Gordon Grau, Director, Sea Grant Hawaii / Professor, University of Hawaii, Manoa, USA
Dr. Tetsuya Hirano, Hawaii Institute of Marine Biology, University of Hawaii, Manoa, USA
Dr. Susumu Hyodo, Ocean Research Institute, University of Tokyo, Japan
Dr. Taisen Iguchi, National Institute for Basic Biology, Japan
Dr. Toyoji Kaneko, University of Tokyo, Japan
Dr. Yoshinao Katsu, National Institute of Basic Biology, Japan
Dr. Steve McCormick, United States Geological Survey, Conte Anadromous Fish Research Center, USA
Dr. Masatoshi Mita, Tokyo Gakugei University, Japan
Dr. Kanta Mizusawa, Kitasato University, Japan
Dr. Shunsuke Moriyama, Kitasato University, Japan
Dr. Tatsuya Sakamoto, Okayama University, Japan
Dr. Larry Riley, California State University, Fresno, USA
Dr. Jennifer Specker, University of Rhode Island, USA
Dr. Craig Sullivan, North Carolina State University, USA
Dr. Lisa Tanguay, University of Alberta, Canada
Dr. Phillip Veillette, University of Rhode Island, USA
Dr. Takashi Yada, National Research Institute of Fisheries Science, Japan
note: lead faculty in bold



Elizabeth Cerny-Chipman, Pomona College, USA
Joseph Chadwick, University of Massachusetts Anadromous Fish Research Center, USA
Sonia Fang, Pomona College
Whitney Janzen, California State University, Fresno, USA
Shizuka Kawatsu, University of Tokyo, Japan
Kyung Mi Lee, University of Tokyo, Japan
Mekuchi Miyuki, University of Tokyo, Japan
Benjamin Reading, North Carolina State University, USA
Andre Seale, Hawaii Institute of Marine BIology, University of Hawaii, Manoa, USA
Sohichiro Takabe, University of Tokyo, Japan
Chiyo Takagi, University of Tokyo, Japan
Hideya Takahashi, University of Tokyo, Japan
Soichi Watanabe, University of Tokyo, Japan
Yoko Yamaguchi, University of Tokyo, Japan



Wed 7/9 Dr. Larry Riley Investigations into ghrelin’s role in regulating fish growth & metabolism
Wed 7/9 Mr. Joe Chadwick Nuclear Receptor Coactivators and Steroid Hormone Action in Brain
Thurs 7/10 Dr. Lisa Tanguay Islet transplantation: Past, present and future directions
Fri 7/11 Dr. Phil Veillette Osmoregulation in summer flounder
Mon 7/14 Dr. Steve McCormick Preparing for the Sea: the Physiology and Endocrinology of Juvenile
     Anadromous Fish
Wed 7/16 Dr. Jennifer Specker Biology and endocrinology of metamorphosis in the summer flounder
Fri 7/18 Dr. Mita Hormonal regulation of oocyte maturation in starfish
Wed 7/23 Dr. Takashi Yada Differential expression of corticosteroid receptor genes in osmoregulatory
     and immune tissues in fish
Thurs 7/24 Dr. Toyoji Kaneko Recent advances in chloride cell research
Thurs 7/24 Ms. Shizuka Kawatsu Shark data logger work
Fri 7/25 Ms. Yoko Yamaguchi Molecular histochemical study on urea reabsorption mechanism in shark kidney 
Fri 7/25 Dr. Susumu Hyodo Recent progress in shark osmoregulation
Mon 7/28 Ms. Miyuki Mekuchi Seawater-acclimated eel evacuate carbonate mineral as “calcium cake”
    for hypoosmoregulation
Mon 7/28 Dr. Soichi Watanabe Osmoreception and water channel in fish
Wed 7/30 Dr. Kyung Mi Lee Eel leptocephali regulate ion and water balances
Wed 7/30 Mr. Hideya Takahashi Osmoregulation in the esophagus of tilapia
Mon 8/4 Dr. Kanta Mizusawa Melanin-concentrating hormone in fish
Wed 8/5 Dr. Craig Sullivan Selective Breeding and Breeding Biotechnology for the Hybrid Striped
     Bass Industry: Status and Directions
Fri 8/8 Dr. Ben Reading Reproduction of white perch: multiple egg yolk precursors (vitellogenins)
    and their receptors
Wed 8/20 Dr. Yoshi Katsu Evolution of estrogen receptors in fishes
Wed 8/20 Dr. Taisen Iguchi Daphnia magna as a model species for endocrine disruption research



Grau EG (2008) Hormones and the environment: Final report the 2008 Edwin W. Pauley Summer Program in Marine Biology. Kaneohe, Hawaii. 14 pp. (Download the report here)



Coming soon. Please send any scanned photos from the Pauley Summer Program to erik.franklin@hawaii.edu.



A list of publications that directly or indirectly resulted from scientific concepts, training, or research experiences during the Pauley Summer Program.

Riley, L.G., Fox, B.K., Breves, J.P., Kaiya, H., Dorough, C.P., Hirano, T., and E.G. Grau, 2008.  Absence of effects of short-term fasting on plasma ghrelin and brain expression of ghrelin receptors in the tilapia, Oreochromis mossambicus. Zool. Sci. 25, 821-827.

Davis, L.K., Visitacion, N., Riley, L.G., Hiramatsu, N., Sullivan, C.V., Hirano, T., Grau, E.G., 2009. Effects of o,p’-DDE, heptachlor, and 17b-estradiol on vitellogenin gene expression and the growth hormone/insulin-like growth factor-I axis in the tilapia, Oreochromis mossambicus. Comparative Biochemistry and Physiology, C., in press.

Uchida, K., Moriyama, S., Breves, J.P., Fox, B.K., Pierce, A.L., Borski, R.J., Hirano, T., Grau, E.G., 2009. cDNA cloning and isolation of somatolactin in Mozambique tilapia and effects of seawater acclimation, confinement stress, and fasting on its pituitary expression. General and Comparative Endocrinology, in press.

 Fox, B.K., Breves, J.P., Hirano, T., Grau, E.G., 2009. Effects of short- and long-term fasting on plasma and stomach ghrelin, and the growth hormone/insulin-like growth factor I axis in the tilapia, Oreochromis mossambicus. Domestic Animal Endocrinology, in press.

Kawatsu, S., Sato, K., Hyodo, S., Watanabe, Y., Breves, J.P., Fox, B.K., Grau, E.G., Miyazaki N., 2008. Swimming behavior of juvenile scalloped hammerhead sharks (Sphyrna lewini ) in response to a predator. Proceeding of Japan Elasmobranch Society, in press.

Watanabe, S., Hirano, T., Grau, E.G., and T. Kaneko (2009): Osmosensitivity of prolactin cells is enhanced in conjunction with increases in the expression of the water channel aquaporin-3 in a euryhaline teleost fish, Mozambique tilapia (Oreochromis mossambicus). American Journal of Physiology, submitted.

Davis, L.K., Fox, B.K., Lim, C., Hiramatsu, N., Sullivan, C.V., Hirano, T., Grau, E.G. Commercial fish diets induce vitellogenesis in male tilapia (Oreochromis mossambicus): a consideration for endocrine disruptor studies. In preparation, to be submitted to Environmental Science and Technology.

Breves, J.P., Hasegawa, S., Yoshioka, M., Fox, B. K., Davis, L.K., Takei, Y., Hirano, T., and E.G. Grau (2009): Osmoregulatory and endocrine responses of two species of cichlid fish, Mozambique tilapia and Nile tilapia, exposed to hyperosmotic environment. In preparation, to be submitted to Comparative Biochemistry and Physiology.

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