Faculty Projects and Publications

Shoemaker, J. M., Riley, L. G., Hirano, T., Grau, E. G., Rubin, D. A., 2005. Differential expression of G6Pase and tip38 in freshwater and saltwater acclimated tilapia: Implications of adaptability for a euryhaline species. Gen. Comp. Endocrinol. submitted.

Seale, A. P., Fiess, J., Hirano, T., Cooke, I., and Grau, E. G. (2004). Disparate release of prolactin and growth hormone from the tilapia pituitary in response to osmotic stimulation and acclimation salinity. Gen. Comp. Endocrinol. submitted.

Seale, A. P., Hirano, T., and Grau, E. G. (2004). Stimulus-secretion coupling in the osmoreceptive prolactin cell of the tilapia. In Mechanosensitivity of the Cells from Various Tissues, A. Kamkin and I. Kiseleva (Eds.), Academic Book, Russian International Publishing House, Moscow, in press.

Kajimura, S., Seale, A. P., Hirano, T., and Grau, E. G. (2004). Physiological concentrations of ouabain rapidly inhibit prolactin release from the tilapia pituitary. Gen. Comp. Endocrinol. 142: 227-233.

Riley, L. G., Fox, B. K., Kaiya, H., Hirano, T., and Grau, E. G. (2004). Long-term treatment of ghrelin stimulates feeding, fat deposition and alters the GH/IGF-I axis in the tilapia, Oreochromis mossambicus. Gen. Comp. Endocrinol. 142: 234-240.

Tipsmark, C. K., Weber, G. M., Strom, C. N., Grau, E. G., Hirano, T., and Borski, R. J. (2004). Involvement of phospholipase C and intracellular calcium signaling in the gonadotropin-releasing hormone regulation of prolactin release from lactotrophs of tilapia (Oreochromis mossambicus). Gen. Comp. Endocrinol. in press.

Shepherd, B. S., Weber, G. M., Vijayan, M. M., Seale, A., Riley, L. G., Richman, N. H. III, Hirano, T. and Grau, E. G. (2004). Control of growth in tilapia: Development and prospect. In Tilapias: Culture, Nutrition and Feeding, C. D. Webster and C. E. Lim (Eds.), Haworth Press, Inc, in press.

Riley, L. G., Hirano, T. and Grau, E. G. (2004). Estradiol-17b and dihydrotestosterone differentially regulate vitellogenin and insulin-like growth factor-1 production in primary hepatocytes of the tilapia, Oreochromis mossambicus. Comp. Biochem. Physiol. 138/2: 177-186.

Weber, G. M., Seale, A. P., Richman III, N. H., Stetson, M. H., Hirano, T. and Grau, E. G. (2004). Hormone release is tied to changes in cell size in the osmoreceptive prolactin cell of a euryhaline teleost fish, the tilapia, Oreochromis mossambicus. Gen. Comp. Endocrinol. 138, 8-13.

Yada, T., Azuma1, T., Schreck, C. B., Hirano, T. and Grau, E.G. (2004). Effects of growth hormone on proliferation and immune function of leucocytes in euryhaline fish. In Trends in Comparative Endocrinologyh Proceedings of Fifth Congress of Asia and Oceania Society for Comparative Endocrinology, T. Oishi, K. Tsutsui, S. Tanaka and S. Kikuyama (Eds.), pp. 95-97.

Seale, A. P. Weber, G. M., Richman III, N. H., Stetson, M. H., Hirano, T. and Grau, E. G. (2004). Cell size regulates hormone release in the osmoreceptive prolactin cell of the euryhaline tilapia, Oreochromis mossambicus. In Trends in Comparative Endocrinologyh Proceedings of Fifth Congress of Asia and Oceania Society for Comparative Endocrinology, T. Oishi, K. Tsutsui, S. Tanaka and S. Kikuyama (Eds.), pp. 138-140.

Seale, A. P., Hirano, T. and Grau, E. G. (2004). Osmoreception: a fish model for a fundamental sensory modality. In Fish Endocrinology, G. Zaccone (Ed.), In press.

Seale, A. P., Cooke, I., Hirano, T. and Grau, E. G. (2004). Evidence that IP3 and ryanodine-sensitive intracellular Ca2+ stores are not involved in acute hyposmotically-induced prolactin release in tilapia. Cell. Physiol. Biochem. 14: 155-166.

Uchida, K., S. Kajimura, T. Yada, T. Hirano, and E. G. Grau (2004). Effects of cortisol on release and gene expression of prolactin and growth hormone in the tilapia, Oreochromis mossambicus. Gen. Comp. Endocrinol. 135: 116-125.

Kajimura, S. Kawaguchi, N., Kaneko, T., Kawazoe, I., Hirano. T., Visitacion, N. Grau, E. G. and Aida, K. (2004). Identification of growth hormone receptor of an advanced teleost, tilapia, with special reference to its expression in the ovary. J. Endocrinol. 181: 65-76.

Kajimura, S., Hirano, T., Moriyama, S., Vakkuri, O., Leppauluoto J. and Grau E. G. (2004). Changes in plasma concentrations of immunoreactive ouabain in the tilapia in response to changing salinity: Is ouabain a hormone in fish? Gen. Comp. Endocrinol. 135: 90-99.

Leedom, T., T. Hirano, and E. G. Grau (2003). Effect of blood withdrawal and angiotensin II on prolactin release in the tilapia, Oreochromis mossambicus. Comp. Biochem. Physiol. 135A: 155-163.

Kaiya, H., M. Kojima, H. Hosoda, L. G. Riley, T. Hirano, E. G. Grau, and K. Kangawa (2003). Amidated fish grelin: purification, cDNA cloning in the Japanese eel and its biological activity. J. Endocrinol. 176: 415-423.

Kaiya, H., M. Kojima, H. Hosoda, L. G. Riley, T. Hirano, E. G. Grau, and K. Kangawa (2003). Identification of tilapia ghrelin and its effects on growth hormone and prolactin release in the tilapia, Oreochromis mossambicus. Comp. Biochem. Physiol. 135BA: 421-429.

Eckert, S. M., T. Hirano, T. A. Leedom, Y. Takei, and E. G. Grau (2003). Effects of angiotensin II and natriuretic peptides on prolactin and growth hormone release in the tlapia, Oreochromis mossambicus. Gen. Comp. Endocrinol. 130: 333-339.

Seale, A. P., N. H. Richman, T. Hirano, I. Cooke, and E. G. Grau (2003). Cell volume increase and extracellular Ca2+ are needed for hyposmotically induced prolactin release in tilapia. Am. J. Physiol. 284: C1280-C1289.

Seale, A. P., N. H. Richman, T. Hirano, I. Cooke, and E. G. Grau (2003). Evidence that signal transduction for osmoreception is mediated by stretch-activated ion 21 channels in tilapia. Am. J. Physiol. 284: C1290-C1296.

Uchida, K., S. Kajimura, L. G. Riley, T. Hirano, K. Aida, and E. G. Grau (2003). Effects of fasting on growth hormone/insulin-like growth factor I axis in the tilapia, Oreochromis mossambicus. Comp. Biochem. Physiol. A 134: 429-439.

Kajimura, S., T. Hirano, N. Visitacion, S. Moriyama, K. Aida, and E. G. Grau (2003). Dual mode of cortisol action on GH/IGF-I/IGFBPs in the tilapia, Oreochromis mossambicus. J. Endocrinol. 178: 91-99.

Collier, J. T., T. Kaneko, T. Hirano and E. G. Grau (2003). Seasonal changes in reproductive activity in the Potter's angelfish (Centropyge potteri) in Kaneohe Bay, Hawaii. Environ. Biol. Fish. 68: 49-57.

Riley, L.G., T. Hirano, and E.G. Grau (2003). Effects of transfer from seawater to fresh water on the growth hormone/insulin-like growth factor-I axis in the tilapia, Oreochromis mossambicus. Comp. Biochem. Physiol. 136: 647-655.

Sparks, R. T., B. S. Shepherd, B. Ron, N. H. Richman III, L. G. Riley, G. K. Iwama, T. Hirano, and E. G. Grau (2003). Effects of environmental salinity and 17a-methyltestosterone on growth and oxygen consumption in the tilapia, Oreochromis mossambicus. Comp. Biochem. Physiol. 136: 657-665.

Riley, L.G., T. Hirano, and E.G. Grau. (2002). Rat ghrelin stimulates growth hormone and prolactin release in the tilapia, Oreochromis mossambicus. Zool. Sci. 19: 797-800.

Kajimura, S., K. Uchida, T. Yada, T. Hirano, K. Aida and E.G. Grau (2002). Effects of insulin-like growth factors (IGF-I and IGF-II) on growth hormone and prolactin release and gene expression in euryhaline tilapia, Oreochromis mossambicus. Gen. Comp. Endocrinol. 123: 223-231

Kaiya, H., M. Kojima, H. Hosoda, L. G. Riley, T. Hirano, E. G. Grau and K. Kanagawa (2002). A novel growth hormone secretagogue, ghrelin: identification and biological activity in fish. Fish. Sci. 68 Suppl I, 749-752.

Moriyama, S., Itoh, T., Takahashi, A., Amano, M., Sower, S. A., Hirano, T., Yamamori, K., and Kawauchi, H. (2002). A homologue of mammalian prolactin-releasing peptide (fish arginyl-phenylalanyl-amide peptide) is a major hypothalamic peptide of prolactin release in teleost fish. Endocrinology 143: 2071-2079.

Riley, L. G., Richman, N. H. III, Hirano, T., and Grau, E. G. (2002). Activation of the growth hormone - IGF-I axis by treatment with 17a-Methyltestosterone and salt water rearing in the tilapia, Oreochromis mossambicus. Gen. Comp. Endocrinol. 127: 285-292.

Seale, A.P., L.G. Riley, T.A. Leedom, S. Kajimura, R.M. Dores, T. Hirano, and E.G. Grau (2002). Effects of environmental osmolality on release of prolactin, growth hormone and ACTH from the tilapia pituitary. Gen. Comp. Endocrinol. 128: 91-101.

Kajimura, S., Moriyama, S., Yada, T., Hirano, T., and Grau, E. G., (2002). Effects of cortisol on GH-IGF-I axis in the euryhaline tilapia, Oreochromis mossambicus. In: R. Keller, H. Dircksen, D. Sedlmeier and H. Vaudrys (Eds.), Proceedings of 21st Conference of European Comparative Endocrinologists. Monduzzi Editore, Bologna, pp. 277-282.

Hirano, T., T.A. Leedom, A.P. Seale, and E.G. Grau (2002). Facilitative effects of angiotensin II on prolactin cell responses to osmotic stimulation in tilapia. In Osmoregulation and Drinking in Aquatic and Terrestrial Vertebrates, (N. Hazon and G. Flik, Eds.), BIOS Scientific Publishers, Oxford: 277-282.

de Jesus, E.G., F.G. Ayson, Y. Amemiya, S. Moriyama, T. Hirano, and H. Kawauchi. (2002). Growth hormone mRNA is expressed during embryonic and early larval stages of milkfish, Chanos chanos. Aquaculture 208: 177-188.

Leedom, T.A., K. Uchida, T. Yada, N.H. Richman III, J.C. Byatt, R.J. Collier, T. Hirano, and E.G. Grau. (2002). Recombinant bovine growth hormone treatment of tilapia: growth response, metabolic clearance, receptor binding and immunoglobulin production. Aquaculture 207: 359-380.

Yada, T., Uchida, K., Kajimura, S., Azuma, T., Hirano, T., and Grau, E. G. (2002). Immunomodulatory effects of prolactin and growth hormone in the tilapia, Oreochromis mossambicus. J. Endocrinol. 173: 483-492.

Seale, A.P., T. Itoh, S. Moriyama, A. Takahashi, H. Kawauchi, T. Sakamoto, M. Fujimoto, L.G. Riley, T. Hirano, and E.G. Grau (2002). Isolation and characterization of a homologue of mammalian prolactin-releasing peptide from the tilapia brain and its effect on prolactin release from the rilapia pituitary. Gen. Comp. Endocrinol. 125: 328-339.

Eckert, S. M., Hirano, T., Yada, T., and Grau, E. G. (2001). Hormonal control of osmoregulation in a stenohaline catfish, Ichtalurus punctatus. In: J. Y. L. Yus (Eds.), Recent Advances in Comparative Endocrinology. Academia Sinica, Taipei, pp. 343-353.

Kajimura, S., K. Uchida, T. Hirano, and E.G. Grau (2001) Effects of recombinant bovine growth hormone on insulin-like growth factor-I gene expression in the euryhaline tilapia, Oreochromis mossambicus. Fish Physiol. Biochem. 25: 221-230.

Eckert, S.M., T. Yada, B.S. Shepherd, M.H. Stetson, T. Hirano, and E.G. Grau (2001). Hormonal control of osmoregulation in the channel catfish Ictalurus punctatus. Gen. Comp. Endocrinol. 122: 270-286.

Shiraishi, K., J. Hiroi, T. Kaneko, M. Matsuda, T. Mori, and T. Hirano (2001). In vitro effect of environmental salinity on chloride cell differentiation in embryos of Mozambique tilapia, Oreochromis mossambicus, measured using a newly developed "yolk-ball" incubation system. J. Exp. Biol. 204: 1883-1888.

Eckert, S. M., Hirano, T., and Grau, E. G. (2001). Hormonal control of osmoregulation in a stenohaline catfish, Ichtalurus punctatus. In Recent Advances in Comparative Endocrinology, (J. Y. L. Yu, Eds.), pp. 343-353. Academia Sinica, Taipei.

Yada, T., T. Azuma, T. Hirano, and E.G. Grau (2001). Efects of hypophysectomy on immune functions in channel catfish. In Perspective in Comparative Endocrinology. Unity and Diversity, (H.J.T. Goos, et al., Eds.), pp. 369-376. Monduzzi Editore, Bologna.

Ayson, F.G., E.G. de Jesus, Y. Amemiya, S. Moriyama, T. Hirano, and H. Kawauchi (2000). Isolation, cDNA cloning, and growth promoting activity of rabbitfish (Siganus guttatus) growth hormone. Gen. Comp. Endocrinol. 117: 251-259.

Shepherd, B.S., S.M. Eckert, I.S. Parhar, M.M. Vijayan, I. Wakabayashi, T. Hirano, E.G. Grau, and T.T. Chen (2000). The hexapeptide KP-102 (D-ala-D-beta-Nal-ala-trp-D-phe-lys-NH2) stimulates growth hormone release in a cichlid fish (Ooreochromis mossambicus). J. Endocrinol. 167: R7-10.

Uchida, K., T. Kaneko, H. Miyazaki, S. Hasegawa, and T. Hirano (2000). Excellent salinity tolerance of Mozambique tilapia (Oreochromis mossambicus): Elevated chloride cell activity in the branchial and opercular epithelia of the fish adapted to concentrated seawater. Zool. Sci. 17: 149-160.