The Losey Lab: Research
In order to test the transmission properties of fish eyes, and the reflectance properties of their colors, we use a spectrophotometer. Basically, a UV-rich light source is passed through the eye, or bounced off the fish, and the resulting photons are picked up by the spectrophotometer, which gives us a graph of percent transmission (or reflectance) vs. wavelength.
In the case of fish eyes, we cut a hole through the back of the eye, up to and including the retina, and then mount the eye on a UV-transparent slide, or one with a hole in it, as shown above (right). We then place the eye on a modified microscope stage, shown to the left. The light source passes through the fiber optic at the top, which is inserted just into the humors, and the light which passes through the eye is picked up below by a collimating lens and passed through another fiber optic to the spectrophotometer. We have compared and verified this system with SubSpec, a submersible spectrophotometry system belonging to Dr. Justin Marshall of the University of Queensland. To learn more about SubSpec or Dr. Marshall's lab, you can visit his website at www.vthrc.uq.edu.au/ecovis.
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