Publications

Hoang, T.T., S.A. Sullivan, J.K. Cusick and H.P. Schweizer. 2002. b-ketoacyl acyl carrier protein reductase (FabG) activity of the fatty acid biosynthetic pathway is a determining factor of 3-oxo-homoserine lactone acyl chain lengths. Microbiology 148: 3849-3856.

Habash, M.B., Beaudette, L.A., Cassidy, M.B., Leung, K.T., Hoang, T., Vogel, H.J., Trevors, J.T., and H. Lee. 2002. Characterization of tetrachlorohydroquinone reductive dehalogenase from Sphingomonas sp. UG30. Biochemical & Biophysical Research Communications 299:634-640.

Hoang, T.T., Kutchma, A.J., Becher, A., and Schweizer, H.P. 2000. A site-specific gene integration system for Pseudomonas aeruginosa. Plasmid 43: 59-72.

Barekzi, N., Beinlich, K.L., Hoang, T.T., Pham, X.-Q., Karkhoff-Schweizer, R.R., and H.P. Schweizer. 2000. The oriC-containing region of the Pseudomonas aeruginosa chromosome undergoes large inversions at high frequency. Journal of Bacteriology 182:7070-7074.

Rungtip, C., Beinlich, K., Hoang, T.T., Becher, A., Karkhoff-Schweizer, R.R., Schweizer, H.P. 2000. Cross-Resistance between Triclosan and Antibiotics in Pseudomonas aeruginosa is Mediated by Multidrug Efflux Pumps: Exposure of a Susceptible Mutant Strain to Triclosan Selects nfxB Mutants Overexpressing MexCD-OprJ. Antimicrobial Agents and Chemotherapy 45(2): 428-432.

Kutchma, A.J., Hoang, T.T., and Schweizer, H.P. 1999. Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-CoA:ACP transacylase (FadD). Journal of Bacteriology 181: 5498-5504.

Hoang, T.T., and Schweizer, H.P. 1999. Characterization of Pseudomonas aeruginosa enoyl-acyl carrier protein reductase (FabI): a target for the antimicrobial triclosan and its role in acylated homoserine lactone synthesis. Journal of Bacteriology 181: 5489-5497.

Hoang, T.T., YuFang Ma, Stern, R.J., McNeil, M.R., and Schweizer, H.P. 1999. Construction and use of low-copy number T7 expression vectors for purification of problem proteins: purification of Mycobacterium tuberculosis RmlD and Pseudomonas aeruginosa LasI and RhlI proteins, and functional analysis of purified RhlI. Gene 237: 361-371.

Hoang, T.T., Karkhoff-Schweizer, R.R., Kutchma, A.J., and Schweizer, H.P. 1998. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212: 77-86.

Handfield, M., Schweizer, H.P., Mahan, M.J., Sanschargrin, F., Hoang, T.T, and Levesque, R.C. 1998. Asd-GFP vectors for in vivo expression technology in Pseudomonas aeruginosa and other Gram-negative bacteria. BioTechniques 24(2): 261-264.

Hoang,T.T and H.P. Schweizer. 1997. Leucine biosynthesis in Pseudomonas aeruginosa: identification and characterization of leuB, encoding 3-isopropylmalate dehydrogenase. Molecular & General Genetics 254: 166-170.

Hoang, T.T., Williams, S., H.P. Schweizer and Lam, J.S. 1997. Molecular genetic analysis of the region containing the essential Pseudomonas aeruginosa asd gene encoding aspartate-b-semialdehyde dehydrogenase. Microbiology 143: 899-907.

Hoang, T.T. and H.P. Schweizer. 1997. Fatty acid biosynthesis in Pseudomonas aeruginosa: cloning and characterization of the fabAB operon encoding b-hydroxydecanoyl-acyl carrier protein dehydratase (FabA) and b-ketoacyl-acyl carrier protein synthase I (FabB). Journal of Bacteriology 179(17): 5326-5332.

Schweizer, H.P. and Hoang, T.T. 1995. An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosa. Gene 158: 15-22.

Book Chapters

Schweizer, H.P., Klassen, T. and T.T. Hoang. 1996. Improved Methods for Gene Analysis and Expression in Pseudomonas. In: Molecular Biology of Pseudomonads (Nakazawa, T., K. Furukawa, D. Haas and S. Silver, eds.), pp. 229-237, ASM Press, Washington, DC.

Schweizer, H.P., T.T. Hoang, K.L. Propst, H. Arvelos and R.R. Karkhoff-Schweizer. 2001. Vector design and development of host systems for Pseudomonas. In: Genetic Engineering: Principle and Methods, Vol. 23, Kluwer Academic/Plenum Publishers, New York, pp. 69-81.

Publications in Preparation

Lu, X., Son, M., and Hoang T. Characterization of the Complete Pentachlorophenol Dechlorination Pathway from Sphingobium chlorophenolicum ATCC 39723 and sp. UG30 in a heterologous host.

Lu, X., Kang, Y., and Hoang T. Utilizing Unique FRTs for Gene-Replacement of Closely Linked Genetic Loci: construction of a double unmarked mutant in the pcpA and pcpE genes of Sphingobium chlorophenolicum strain ATCC 39723.

Son, M., Nguyen, D., Lu, X., Hoang, T. Engineering of Suicidal Conjugation-Proficient E. coli Donors for Easy Selection of Transconjugants: increasing the host-range for RP4-based conjugation.

Son, M., Nguyen, Kang, Y., and Hoang, T. Engineering of a Broad-Host-Range Genetic Construct for Protein Expression in Bacteria Based on the Phage T7 Gene 1 System.

Nguyen, D., Son, M., and Hoang, T. Inverse Regulation of Fatty Acid Biosynthesis and Degradation in Pseudomonas aeruginosa by Long Chain Fatty Acids.

Son, M., Beversdorf, L. Nguyen, D., and Hoang, T. Analyses of Two Acyl-CoA Synthases of Pseudomonas aeruginosa involved in Fatty Acid Degradation.

Son, M., Nguyen, D., Lu, X., Hoang, T. Examining the Whole Genome Expression Profile of Pseudomonas aeruginosa during Lung Surfactant Component Phosphatidylcholine Degradation.

Beversdorf, L., Son, M., Nguyen, D., Lu, X., Hoang, T. Kinetic Characterization of P. aeruginosa Acyl-CoA Synthases.