College of Natural Sciences

Department of Microbiology

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1. Kang, Y., Norris, M., Zarzycki-Siek, J., Donachie, S.P., Nierman, W.C., and Hoang, T.T. 2011. Transcript amplification from single bacterium for transcriptome analysis. Genome Research 21: 925-935.

2. Kang, Y., Norris, M., Wilcox, B.A., Tuanyok, A., Keim, P.S., and Hoang, T.T. 2011. Knockout and pullout recombineering for naturally transformable Burkholderia thailandensis and Burkholderia pseudomallei. Nature Protocols 6(8): 1085-1104.

3. Norris MH, Kang Y, Wilcox B, Hoang TT. 2010. Stable site-specific fluorescent tagging constructs optimized for Burkholderia species. Applied Environmental Microbiology 76(22):763540.

4. Kang Y, Zarzycki-Siek J, Walton CB, Norris MH, Hoang TT. 2010. Multiple FadD acyl-CoA synthetases contribute to differential fatty acid degradation and virulence in Pseudomonas aeruginosa. PLoS ONE 5(10):e13557.

5. Norris, M.H., Kang, Y., Lu, D., Wilcox, B.A., and Hoang T.T. 2009. Glyphosate Resistance as a Novel Select-Agent-Compliant Non-Antibiotic Selectable Marker in Chromosomal Mutagenesis of the Essential Genes asd and dapB of Burkholderia pseudomallei . Applied and Environmental Microbiology 75(19):6062-6075.

6. Kang, Y., Lunin, V.V., Skarina, T., J., Savchenko, A., Schurr, M.J., and Hoang, T.T. 2009. The long-chain fatty acid sensor, PsrA, modulate the expression of rpoS and the type III secretion exsCEBA-operon in Pseudomonas aeruginosa. Molecular Microbiology 73(1):120-136.

7. Kang, Y., Norris, M.H., Barrett, A.R., Wilcox, B.A. and Hoang, T.T. 2009. Engineering of tellurite-resistant genetic tools for single-copy chromosomal analysis of Burkholderia spp. and characterization of the B. thailandensis betBA-operon. Applied and Environmental Microbiology 75(12):4015-4027.

8. Barrett, R.B., Kang, Y., Inamasu, K.S., Son, M.S., Vukovich, J.M., and T.T. Hoang. 2008. Genetic tools for allelic-replacement in Burkholderia species. Applied and Environmental Microbiology 74(14):4498-4508.

9. Kang, Y., D. T. Nguyen, M. S. Son, and T. T. Hoang. 2008. The Pseudomonas aeruginosa PsrA responds to long-chain fatty acid signals to regulate the fadBA5 ß-oxidation operon. Microbiology 145(6):1584-1596.

10. Son, S.S., Nguyen, D.T., Kang, Y., and T.T. Hoang. 2008. Engineering of FRT-lacZ fusion constructs: induction of the Pseudomonas aeruginosa fadAB1 operon by medium and long chain-length fatty acids. Plasmid 59(2):111-118.

10. Son, M.S., Matthews Jr., W.J., Kang, Y. Nguyen, D.T., and T.T. Hoang. 2007. In vivo evidence of Pseudomonas aeruginosa nutrient acquisition and pathogenesis in the lungs of cystic fibrosis patients. Infection and Immunity 75(11):5313-5324.

11. Kang, Y., Son, M.S., and T.T. Hoang. 2007. One step engineering of T7-expression strains for protein production: increasing the host-range of T7-expression system. Protein Expression and Purification 55: 325-333.

12. Yamniuk, A.P., Nguyen, L.T., Hoang, T.T., and Vogel, H.J. 2004. Metal ion binding properties and conformational states of calcium- and integrin-binding protein. Biochemistry 43: 2558-2568.

13. Hoang, T.T., S.A. Sullivan, J.K. Cusick and H.P. Schweizer. 2002. ß-ketoacyl acyl carrier protein reductase (FabG) activity of the fatty acid biosynthetic pathway is a determining factor of 3-oxo-homoserine lactone acyl chain lengths. Microbiology 148: 3849-3856.

14. Habash, M.B., Beaudette, L.A., Cassidy, M.B., Leung, K.T., Hoang, T., Vogel, H.J., Trevors, J.T., and H. Lee. 2002. Characterization of tetrachlorohydroquinone reductive dehalogenase from Sphingomonas sp. UG30. Biochemical & Biophysical Research Communications 299:634-640.

15. Hoang, T.T., Kutchma, A.J., Becher, A., and Schweizer, H.P. 2000. A site-specific gene integration system for Pseudomonas aeruginosa. Plasmid 43: 59-72.

16. Barekzi, N., Beinlich, K.L., Hoang, T.T., Pham, X.-Q., Karkhoff-Schweizer, R.R., and H.P. Schweizer. 2000. The oriC-containing region of the Pseudomonas aeruginosa chromosome undergoes large inversions at high frequency. Journal of Bacteriology 182:7070-7074.

17. Rungtip, C., Beinlich, K., Hoang, T.T., Becher, A., Karkhoff-Schweizer, R.R., Schweizer, H.P. 2000. Cross-Resistance between Triclosan and Antibiotics in Pseudomonas aeruginosa is Mediated by Multidrug Efflux Pumps: Exposure of a Susceptible Mutant Strain to Triclosan Selects nfxB Mutants Overexpressing MexCD-OprJ. Antimicrobial Agents and Chemotherapy 45(2): 428-432.

18. Kutchma, A.J., Hoang, T.T., and Schweizer, H.P. 1999. Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-CoA:ACP transacylase (FadD). Journal of Bacteriology 181: 5498-5504.

19. Hoang, T.T., and Schweizer, H.P. 1999. Characterization of Pseudomonas aeruginosa enoyl-acyl carrier protein reductase (FabI): a target for the antimicrobial triclosan and its role in acylated homoserine lactone synthesis. Journal of Bacteriology 181: 5489-5497.

20. Hoang, T.T., YuFang Ma, Stern, R.J., McNeil, M.R., and Schweizer, H.P. 1999. Construction and use of low-copy number T7 expression vectors for purification of problem proteins: purification of Mycobacterium tuberculosis RmlD and Pseudomonas aeruginosa LasI and RhlI proteins, and functional analysis of purified RhlI. Gene 237: 361-371.

21. Hoang, T.T., Karkhoff-Schweizer, R.R., Kutchma, A.J., and Schweizer, H.P. 1998. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212: 77-86.

22. Handfield, M., Schweizer, H.P., Mahan, M.J., Sanschargrin, F., Hoang, T.T, and Levesque, R.C. 1998. Asd-GFP vectors for in vivo expression technology in Pseudomonas aeruginosa and other Gram-negative bacteria. BioTechniques 24(2): 261-264.

23. Hoang,T.T and H.P. Schweizer. 1997. Leucine biosynthesis in Pseudomonas aeruginosa: identification and characterization of leuB, encoding 3-isopropylmalate dehydrogenase. Molecular & General Genetics 254: 166-170.

24. Hoang, T.T., Williams, S., H.P. Schweizer and Lam, J.S. 1997. Molecular genetic analysis of the region containing the essential Pseudomonas aeruginosa asd gene encoding aspartate-b-semialdehyde dehydrogenase. Microbiology 143: 899-907.

25. Hoang, T.T. and H.P. Schweizer. 1997. Fatty acid biosynthesis in Pseudomonas aeruginosa: cloning and characterization of the fabAB operon encoding ß-hydroxydecanoyl-acyl carrier protein dehydratase (FabA) and ß-ketoacyl-acyl carrier protein synthase I (FabB). Journal of Bacteriology 179(17): 5326-5332.

26. Schweizer, H.P. and Hoang, T.T. 1995. An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosa. Gene 158: 15-22.

Book Chapters

1. Schweizer, H.P., Klassen, T. and T.T. Hoang. 1996. Improved Methods for Gene Analysis and Expression in Pseudomonas. In: Molecular Biology of Pseudomonads (Nakazawa, T., K. Furukawa, D. Haas and S. Silver, eds.), pp. 229-237, ASM Press, Washington, DC.

2. Schweizer, H.P., T.T. Hoang, K.L. Propst, H. Arvelos and R.R. Karkhoff-Schweizer. 2001. Vector design and development of host systems for Pseudomonas. In: Genetic Engineering: Principle and Methods, Vol. 23, Kluwer Academic/Plenum Publishers, New York, pp. 69-81.

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