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Last updated

July 25, 2003
 
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Protocols used in the L2TR (Idiomarina loihiensis) Genome Sequencing
     
Overall genome sequencing strategy

Genomic DNA isolation (gram negative)

Genomic DNA isolation (gram positive)

Random Shotgun Library construction protocol

DNA Blunting protocol

DNA Ligation protocol

Electroporation protocol

96-well plasmid prep protocol

Beckman CEQ Automated sequencing protocol (short version)

Beckman CEQ Automated sequencing protocol (long version)

Genome assembly protocol (under construction)

Long PCR protocol

     

Equipment used

 

   
   
Beckman T-25 centrifuge Bio-Rad iCycler PCR machine    
   
   
Beckman CEQ 8000 sequencer Silicon Graphics's Origin 2000 with Ciprico disc arrays    

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