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August 10, 2003
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PCR Purification [back]  

PCR Product Purification (from Qiagen QIAquick PCR Purification Kit)

  1. Add 5 volumes of PB Buffer to 1 volume of PCR reaction and mix
  2. Transfer to QIAquick spin column (purple column)
  3. Centrifuge for 1 minute
  4. Discard low-through
  5. Add 0.75 mL of PE Buffer and centrifuge for 1 minute
  6. Discard low-through
  7. Centrifuge for 1 minute
  8. Discard low-through
  9. Repeat steps 7-8
  10. Place QIAquick (purple) column in clean eppendorf tube and dry completely
  11. Elute with 25-30 mL of Elution Buffer or HPLC water


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