Main Title Image

Last updated

August 10, 2003
 
IntroductionResearch PagesPeople involvedPhoto galleriesPublicationsOther sample sitesEducationLinks to other micobial observatories
PCR Purification [back]  

PCR Product Purification (from Qiagen QIAquick PCR Purification Kit)

  1. Add 5 volumes of PB Buffer to 1 volume of PCR reaction and mix
  2. Transfer to QIAquick spin column (purple column)
  3. Centrifuge for 1 minute
  4. Discard low-through
  5. Add 0.75 mL of PE Buffer and centrifuge for 1 minute
  6. Discard low-through
  7. Centrifuge for 1 minute
  8. Discard low-through
  9. Repeat steps 7-8
  10. Place QIAquick (purple) column in clean eppendorf tube and dry completely
  11. Elute with 25-30 mL of Elution Buffer or HPLC water


   

  This website is maintained by Alam's Lab, Department of Microbiology, University of Hawai'i. All rights reserved.

Link to National Science Foundation website Link to University of Hawaii website Link to Department of Microbiology website Link to Maqsudul Alam lab website Back to main page