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August 10, 2003
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Anchialine pond at Southeast Island, Pearl and Hermes Atoll [back]      

Pearl and Hermes Water Chemistry

Water temperature and salinity in the Southeast Island pond were 24.6°C and 26.9 ppt, respectively. Chl a measured 0.4 mg/L. The H2S concentration was 0.6 mM and total N and DON were 164.0 and 111.1 mM, respectively. The NO3- concentration (48.0 mM) exceeded by almost an order of magnitude that of NH4+ (4.9 mM). Over 99% of the total phosphorus (41.30 mM) comprised SRP; SNP was less than 1% of TP. The SRSi concentration was 14.05 mM.



Pearl and Hermes Cultures

Twenty-nine pure cultures of bacteria from Southeast Island (designated PH) affiliated with 18 taxonomically distinct strains from 10 families within the a- and g-Proteobacteria, the Firmicutes and Cyanobacteria (Table1).

Three cultures from Southeast Island shared <97% 16S rRNA identities with cultivated strains in the literature (Table). PH27A is a highly motile, Gram negative rod that forms a distinct lineage within the Halomonadaceae; PH35 is similarly removed (91.58% 16S rRNA identity) from Serratia proteomaculans, its nearest described neighbor. One of three a-Proteobacteria from Pearl and Hermes (PH30) will be described as a new member of the genus Thalassospira (Rhodospirillaceae).

Pearl and Hermes Clones Table
Taxon OTU Representative clone NT Closest relative Homology (%) Family/Group Accession #
a-Proteobacteria 1 PH-B13 1426 a-Proteobacterium AJ133762 1361/1413 (96.32) Rhodobacteraceae AF513936
g-Proteobacteria 2 PH-B39N 1497 Aeromonas bestiarum X60406 1491/1497 (99.60) Aeromonadaceae AF513938
Firmicutes 3 PH-B53N 1514 Bacillus sp. AB066347 1500/1506 (99.60) Bacillaceae AF513963
Pearl and Hermes Cultures Table
Taxon Culture NT Closest relative Homology (%) Family/Group Accession #
a-Proteobacteria PH30 1396 a-Proteobacterium AB024595 1332/1361 (97.87) Rhodospirillaceae  AF513476
  PH33 1363 Rhodovulum sulfidophilum D16422 1347/1348 (99.93) Rhodobacteraceae  AF513477
  PH34B, PH34P 1364 Rhodobium marinum D30791 1354/1355 (99.93) Rhizobiaceae  AF513478
g-Proteobacteria PH1 1447 Vibrio orientalis X74719 1395/1428 (97.69) Vibrionaceae  AF513461
  PH5 1431 Alteromonas alvinellae AF288360 1416/1422 (99.58) Alteromonadaceae  AF513462
  PH8, PH2, PH6, PH7, PH9, PH22,PH24, PH26 1464 Vibrio proteolyticus X74723 1421/1432 (99.23) Vibrionaceae  AF513463
  PH17, PH15 1442 Halomonas marisflavi AF251143 1421/1437 (98.89) Halomonadaceae  AF513464
  PH19 1433 Vibrio furnissii X76336 1337/1368 (97.73) Vibrionaceae  AF513465
  PH25, PH3 1448 Salinivibrio vallismortis AF057016 1428/1439 (99.24) Vibrionaceae  AF513466
  PH27A 1436 Oceanospirillum maris AB006763 1151/1252 (91.93) Halomonadaceae  AF513467
  PH29 1415 Uncultured soil bacterium AF423262 1406/1416 (99.29) Enterobacteriaceae  AF513468
  PH31, PH34W 1435 Serratia marcesens AF124038 1408/1413 (99.65) Enterobacteriaceae  AF513469
  PH35 1457 Uncultured Proteobacterium AJ310680 1339/1461 (91.65) Aeromonadaceae  AF513470
  PH39 1440 Shewanella sp. AJ319767 1425/1429 (99.72) Alteromonadaceae  AF513471
Firmicutes PH28 1383 Halobacillus trueperi AJ310149 1366/1377 (99.20) Bacillaceae  AF513472
  PH27B 1443 Bacillus cereus AF290548 1424/1426 (99.86) Bacillaceae  AF513473
  PH32 1349 Bacillus sp. AB017591 1332/1342 (99.25) Bacillaceae  AF513474
Cyanobacteria PH40 1480 Synechococcus sp. AJ000716 1427/1434 (99.51) Chroococcales  AF513475

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