James T. Douglas

James Douglas

Professor

We are studying the pathogenesis of infectious diseases, with our major emphasis on tuberculosis, through molecular epidemiology by working with clinical isolates or their DNA from samples collected through out the Western Pacific. We also continue use and develop serodiagnostic tests for these difficult to diagnose bacterial diseases. These investigations require detection and recognition of specific molecules detected by their DNA sequences, monoclonal antibodies, or patient sera. Analysis of frequency and distribution of specific markers of pathogenesis associated with clinical isolates provides insight on the biology of these infectious diseases. In addition to these efforts, we also are evaluating potential antibiotics from environmental organisms.

jtd379@gmail.com
808-956-4639
Snyder 101E

Current Research

Bacterial Diseases: Tuberculosis, Leprosy, Brucellosis; Leptospirosis. We are studying the pathogenesis of infectious diseases, with our major emphasis on tuberculosis, through molecular epidemiology by working with clinical isolates or their DNA from samples collected through out the Western Pacific. We also continue use and develop serodiagnostic tests for these difficult to diagnose bacterial diseases. These investigations require detection and recognition of specific molecules detected by their DNA sequences, monoclonal antibodies, or patient sera. Analysis of frequency and distribution of specific markers of pathogenesis associated with clinical isolates provides insight on the biology of these infectious diseases. In addition to these efforts, we also are evaluating potential antibiotics from environmental organisms.


Selected Publications

  1. Barihuta, T., Rigouts, L., Barette, M., Colllard, J. P., De Bruyn, J., Kadende, P., Kamamfu, G., Douglas, J. T., and Portaels, F. (1993) Rapid, early and specific diagnosis of tuberculosis and other mycobacterial diseases in Burundi. Ann. Soc. Belg. Med. Trop. 73(Suppl. 1):41-51.
  2. Van Soolingen, D., Qian, L., de Haas, P.E.W., Douglas, J.T., Enkhasaikan, D., and van Embden, J.D.A. (1995) Predominance of a single clone of Mycobacterium tuberculosis in China and other countries in South East Asia. J. Clin. Microbiol. 33:3234-3238.
  3. Van Embden, J.D.A., de Haas, P., Heersma, H., Schouls, L., Douglas, J.T., Qian, L., Herman, P.W.M., Messadi, F., Portaels, F., and van Sooligen, D. (1995) Repetitive DNA as a tool to study the genetic variation and epidemiology Mycobacterium tuberculosis. “Ecology of pathogenic bacteria: molecular and evolutionary aspects”. Academy of Science, The Netherlands.
  4. Qian, L., van Embden, J. D. A., vd. Zanden, A. G. M., Weltevereden, E. F., Duanmu, H., and Douglas, J. T. (1999) Retrospective analysis of the Beijing family of Mycobacterium tuberculosis in preserved lung tissues. J. Clin. Microbiol. 37:471-474.
  5. Qian, L., Abe, C., Lin, T., Yu, M. C., Cho, S.N. and Douglas. J. T. (2002). rpoB Genotypes in Drug-Resistant Mycobacterium tuberculosis Isolates from East Asian Countries. J. Clin. Microbiol. 40:1091-1094.
  6. Douglas, J. T., Qian, L., Montoya, J. C., Musser, J. M., van Embden, J. D. A., van Soolingen, D. and Kremer, K. (2003). Characterization of the Manila Family of Mycobacterium tuberculosis. J. Clin. Microbiol. 41:2723-2726.
  7. Walton, C. B., Inos, A. B. H., Andres, O. A., Jube, S., Gert de Couet, H., Douglas, J. T., Patek, P. Q., Borthakur, D. (2008). Immunization with hybrid recombinant Mycobacterium tuberculosis H37Rv proteins increases the TH1 cytokine response in mice following a pulmonary instillation of irradiated mycobacteria. Vaccine 26: 4396-4402.
  8. Mizanbayeva, S., Smits, H. L., Zhalilova, K., Theresia, H., Abdoel, T. H., Kozakov, S., Ospanov, K. S., Elzer, P.H. and Douglas, J. T. (2009). Diagnosis of human brucellosis in Kazakhstan: application of a user-friendly diagnostic assay. Diagnostic Microbiology and Infectious Disease.
  9. Frink, S., Qian, L., Yu, S., Cruz, L., Desmond, E. and Douglas, J.T. (2011). Rapid Deletion-Based Subtyping System for the Manila Family of Mycobacterium tuberculosis. Clinical Microbiology 49: 1951-1955.
  10. Hoshide, M., Qian, L., Rodrigues, C., Warren, R, Victor, T., Evasco II, H.B., Tupasi, T., Crudu, T., and Douglas, J. T. (2014). Geographical Differences Associated with SNPs in Nine Gene Targets among Resistant Clinical Isolates of Mycobacterium tuberculosis. J. Clin Microbiol. 52(5):1322. DOI: 10.1128/JCM.00857-13.
  11. Rodwell, T., Valafar, F., Douglas, J. T., Qian, L., Garfein, R., Chawla, A., Torres, J., Zadorozhny, V., Kim, M. S., Hoshide, M., Catanzaro, D., Jackson, L., S.-Y. Grace Lin, S-Y. G., Desmond, E., Rodrigues, C., Eisenach, K., Victor, T., Crudu, V., Ismail, N., Gler, M.T. and Catanzaro, A. (2014) Predicting Extensively Drug-resistant Tuberculosis (XDR-TB) Phenotypes with Genetic Mutations. J. Clin. Microbiol. 52(3): 3781-3789.
Department of Microbiology
2538 McCarthy Mall, Snyder 207
uhmicro@hawaii.edu
808.956.8553
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