JABSOM Tropical Medicine Seminar

September 29, 12:00pm - 1:00pm
Mānoa Campus, JABSOM Kakaako Campus, via Zoom Add to Calendar

TRMD 690 Wednesday, September 29, 2021 via Zoom @ 12:00 PM - 1:00 PM *****Zoom Link: https://zoom.us/j/97962975142?pwd=aFdJZmhrbjdDOGQ2UFZHZTYyeDhUZz09 Meeting ID: 979 6297 5142 Passcode: 306033*****"Development of Strand-Specific PCR to Detect Replicating SARS-CoV-2 in COVID-19 Patients"*****Abstract: Testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an essential diagnostic tool for monitoring the ongoing COVID-19 pandemic. Currently, the gold standard for the diagnosis of acute SARS-CoV-2 infection is quantitative reverse transcription polymerase chain reaction (qRT-PCR), which is based on nucleic acid amplification technology and targets the genome of SARS-CoV-2. Despite the widespread use of qRT-PCR in clinical settings, it cannot differentiate between replicating and non-replicating virus. Therefore, we aim to develop a strand-specific qRT-PCR multiplex assay that can detect both positive- and negative-sense replicating RNA strands that are synthesized during SARS-CoV-2 infection. With this assay, we can determine the infectiousness of symptomatic and asymptomatic COVID-19 patients and ultimately aid in clinical decision-making for patients who are positive for SARS-CoV-2*****Alanna Tseng, Ph.D., Junior Research Scientist, Department of Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa

Event Sponsor
JABSOM Tropical Medicine, Mānoa Campus

More Information
Vivek Nerurkar, Ph.D., 808-692-1668, nerurkar@hawaii.edu, TRMD 690_Alanna Tseng (PDF)

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